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Wholesale EGF Epidermal growth factor online 100mcg/vial

US$8.20

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buy EGF online from us from us.buy EGF (Epidermal Growth Factor) online at discount price,Epidermal growth factor is a low-molecular-weight polypeptide first purified from the mouse submandibular gland.
What is Epidermal Growth Factor (EGF) ?
Epidermal Growth Factor (EGF) is a small mitogenic polypeptide present throughout a large number of tissues and body fluids in many mammalian species. EGF is a member of a growth factor family characterized by 6 conserved cysteine motifs that form three disulfide bonds. Human EGF is an identical molecule to ß-urogastrone, a molecule isolated on the basis of its ability to inhibit gastric acid secretion. EGF is structurally homologous to human transforming growth factor-a, and both exert their actions through EGF receptors.

Analysis Note for Epidermal Growth Factor
The biological activity of this product is measured in a proliferation assay using BALB/MK cells. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.|

Biochem/physiol Actions for EGF
EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, vascular and corneal endothelial cells, bovine granulosa, HeLa cells, SV40-3T3 cells and mammary epithelial cells. Stimulation of ion fluxes, glucose transport, glycolysis, and synthesis of DNA, RNA and proteins are all cellular metabolic effects of EGF. EGF also plays a biological role in inhibition of gastric acid secretion, support of growth during fetal development and neuromodulation in the central nervous system.

Caution for Epidermal Growth Factor
This product should be stored at -20°C and will retain activity for two years. After reconstitution, it can be stored at 2-8°C for one month, or frozen in aliquots at -70°C or -20°C for longer periods.

Preparation Note
This product was lyophilized from a 0.2 μm-filtered solution of phosphate buffered saline, at pH 7.4. It should be reconstituted by adding the contents of the vial to 1 mg/mL using 10 mM acetic acid. To dilute to lower concentrations, but no fewer than 10 μg/mL, an addition of 0.1% BSA or HSA will be needed. For use in proliferation assays, further dilute the sample with medium without albumin.

Application with EGF
This product is used as a mitogen, triggering the cell to commence mitosis, in a variety of cell lines. In tissue cultures, EGFacts to reduce or eliminate the requirement for serum and can be used in conjunction with other media additives and hormone.


  • Model: EGF 100mcg
  • 59330 Units in Stock


Current Reviews: 85

This product was added to our catalog on Wednesday 01 June, 2011.

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